Referência:
Macedo HT, Rentas MF, Vendramini THA, Macegoza MV, Amaral AR, Jeremias JT, de Carvalho Balieiro JC, Pfrimer K, Ferriolli E, Pontieri CFF, Brunetto MA. Weight-loss in obese dogs promotes important shifts in fecal microbiota profile to the extent of resembling microbiota of lean dogs. Anim Microbiome. 2022 Jan 6;4(1):6. doi: 10.1186/s42523-021-00160-x. PMID: 34991726; PMCID: PMC8740440.
Resumo:
mong the undesirable changes associated with obesity, one possibility recently raised is dysbiosis of the intestinal microbiota. Studies have shown changes in microbiota in obese rats and humans, but there are still few studies that characterize and compare the fecal microbiota of lean, obese and dogs after weight loss. Thus, this study aimed to evaluate the effects of a weight loss program (WLP) in fecal microbiota of dogs in addition to comparing them with those of lean dogs. Twenty female dogs of different breeds, aged between 1 and 9 years were selected. They were equally divided into two groups: Obese group (OG), with body condition score (BCS) 8 or 9/9, and body fat percentage greater than 30%, determined by the deuterium isotope dilution method, and lean group (LG) with BCS 5/9, and maximum body fat of 15%. Weight loss group (WLG) was composed by OG after loss of 20% of their current body weight. Fecal samples were collected from the three experimental groups. Total DNA was extracted from the feces and these were sequenced by the Illumina methodology. The observed abundances were evaluated using a generalized linear model, considering binomial distribution and using the logit link function in SAS (p < 0.05).
Palavras-chave: obesidade, desbiose, trato gastrointestinal, microbiota